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The HIV-1 viral accessory protein Vif prevents the encapsidation of the antiviral cellular cytidine deaminases APOBEC3F and APOBEC3G by inducing their proteasomal degradation. In the absence of Vif, APOBEC3G is encapsidated and blocks virus replication by deaminating cytosines of the viral cDNA. APOBEC3G encapsidation has been recently shown to depend on the viral nucleocapsid protein; however, the...
The initiation of reverse transcription of human immunodeficiency virus type 1 (HIV-1) exclusively utilizes tRNA Lys,3 as a primer. Previous studies have shown that HIV-1 could use alternative tRNAs, such as tRNA Ile or tRNA His , to initiate reverse transcription only if the primer binding site (PBS) was made complementary to the 3′ terminal 18 nucleotides of the cognate tRNA...
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