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Prostate cancer (PCa) is a leading cause of cancer-related death among males globally. To date, prostate-specific antigen (PSA), as a typical tumour marker, has been widely used in the early diagnosis of PCa. However, in practical clinical tests, high serum levels of PSA show a high probability for false-positive results, leading to misdiagnoses. In this study, we developed a new classification system...
A sensitive Ag+ sensor based on nucleic acid probes modified silica microfiber interferometry is designed and developed. The probes on microfiber surface plays the part on catching Ag+ as tentacles, while their conformation change from random coils to hairpins. It induces the fiber surface refractive index change, which is captured by the optical fiber and translated into a significant wavelength...
A novel “in-electrode”-type electrochemiluminescence (ECL) immunosensor for the sensitive detection of squamous cell carcinoma antigen (SCCA) was constructed using magnetic graphene oxide (nanoFe3O4@GO) and Au nanoparticles/graphitic-phase carbon nitride (AuNPs/g-C3N4). The capture probe was prepared by immobilizing the primary antibody of SCCA (Ab1) on the nanoFe3O4@GO, while the AuNPs/g-C3N4 nanocomposites...
A label-free electrochemiluminescence (ECL) immunosensor for ultrasensitive and rapid detection of marine pathogenic bacterium Vibrio parahaemolyticus (VP) in seawater and seafood was developed based on multi-functionalized graphene oxide, which was prepared with N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and VP antibody (anti-VP) simultaneously immobilized on the surface of magnetic graphene oxide...
A fully automated method for the detection of four tobacco-specific nitrosamines (TSNAs) in mainstream cigarette smoke (MSS) has been developed. The new developed method is based on two-dimensional online solid-phase extraction–liquid chromatography–tandem mass spectrometry (SPE/LC–MS/MS). The two dimensional SPE was performed in the method utilizing two cartridges with different extraction mechanisms...
A super-high-sensitivity immunoassay based on surface-enhanced Raman scattering (SERS) was implemented using the nano-Au immune probes and nano-Ag immune substrate. Ultraviolet–visible extinction spectra, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) images, and SERS spectra were used to characterise the nano-Au immune probes and the nano-Ag immune substrate. The nano-Ag...
Three anion-exchangeable, silica-confined ionic liquids were synthesized for solid phase extraction of lactic acid from fermentation broth, followed by high-performance liquid chromatography coupled to ultraviolet detection. By comparing the adsorption isotherms of lactic acid on different silica-confined ionic liquids, interactions between the lactic acid and sorbents were investigated. The adsorbed...
Generally speaking, measurement of hydroxylated radical products of salicylic acid requires a fussy separation process. In this study, we describe a simple method to electrochemically detect hydroxyl radicals (OH) using 4-hydroxybenzoic acid (4-HBA) as the OH trap. The OH is generated by the Fenton reaction from iron (II) sulfate and hydrogen peroxide in a phosphate buffer solution. Experimental results...
An immunosensing system for Schistosoma Japonicum antibody (SjAb) assay has been developed which is useful for the diagnosis of schistosomaisis. To circumvent the difficulty of regeneration of the immunosensing device, the sol-gel technique is used which results in a considerable retention of the activity of the encapsulated antigen (SjAb) and easily diffusing into the pores of the polymeric silica...
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