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The transfection efficiency of siRNA mediated by cationic polymers is limited due to the instability of polymers/siRNA complexes in the presence of serum. Poly(ethylene glycol) (PEG) is usually applied to modify cationic polymers, so as to reduce protein and cell adsorption and then to improve siRNA transfection efficiency. However, the polymers’ modification with PEG mostly consumes the free amino...
Nanoassemblies for cancer gene therapy are designed by Peifeng Liu and co‐workers, as described in article number 2003523, with enhanced serum resistance and robust siRNA transfection efficiency in serum. In vivo, the nanoassemblies, with high stabilities in serum, protect siRNA from degradation and reach tumor sites efficiently.
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