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Cytosolic pyruvate kinase (PK c ) from leaves of the castor oil plant (Ricinus communisL.) has been purified 3900-fold to apparent homogeneity and a final specific activity of 51 μmol of pyruvate produced/min/mg protein. PAGE, immunoblot, and gel filtration analyses of the final preparation indicated that this enzyme is an α 2 β 2 heterotetramer of about 250 kDa that is composed...
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