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Fed-batch cultures of Nocardia sp. were carried out in a 5-l fermentor for production of cis-epoxysuccinate hydrolase (EH). Since EH production was significantly repressed by glucose, the cultivation process was divided into a growth phase on glucose and a production phase induced by cis-epoxysuccinate (ES). The cells of Nocardia sp. were unable to utilize exogenous tartrate, so the intracellular...
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