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Affinity chromatography, as one of the important tools for studying the binding affinity between ligands and receptors, plays a key role in the process of screening and analyzing active compounds. Cell membrane chromatography (CMC) utilizes cell membrane receptors as stationary phases to perform screening and binding affinity of compounds with specific receptors whose three-dimensional configurations...
The aim of this paper was to develop an analytical method for determining the levels of the Benzo[a]pyrene (B[a]P) metabolites, 3-hydroxybenzo(a)pyrene (3-OHB[a]P), and (+)- anti -benzo(a)pyrene diol-epoxide( BPDE, combined with DNA to formate adducts) in the blood and brain tissue of B[a]P-exposed rats using high-performance liquid chromatography coupled with fluorescence detection...
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