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Background
Testicular cancer (TC) is the most common cancer diagnosed in men of reproductive age group. Sperm banking is recommended in these patients prior to cancer treatment. There is no literature describing the proteins dysregulated in the spermatozoa of TC patients with poor motility.
Objective
The primary objective of this study was to compare the differences in the sperm proteome of normozoospermic...
Oxidation–reduction potential describes the balance between the oxidants and antioxidants in fluids including semen. Various artificial culture media are used in andrology and IVF laboratories for sperm preparation and to support the development of fertilized oocytes under in vitro conditions. The composition and conditions of these media are vital for optimal functioning of the gametes. Currently,...
Semen hyperviscosity delays the liquefaction of semen sample and is subjected to limited proteolysis by addition of α‐chymotrypsin to reduce the viscosity. α‐Chymotrypsin is a proteolytic enzyme involved in degradation of the proteins and polypeptides. Even though α‐chymotrypsin improves the handling of hyperviscous samples, its effect on the sperm proteins is not clear. This study was aimed to evaluate...
Seminal oxidative stress (OS) is well‐known to affect male fertility status. The discrepancy in OS measurement has hindered its clinical use as a quality indicator for semen. Some tests measured single markers of oxidants or reductants, leading to lack of standardization of results. Oxidation–reduction potential (ORP) is a better representative for OS as it provides an overall measure of the activity...
One of the challenges with the sperm DNA fragmentation results is the inconsistency and the large variability in the results obtained by different techniques. The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay quantifies the incorporation of fluoresceinated dUTP into single‐ and double‐strand DNA breaks by labeling the 3′‐OH terminal with TdT. The goal of this study was...
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