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The lac operon is a delicate inducible gene expression element in bacteria. To efficiently induce gene expression, a sufficient dosage of an inducer, usually that of 500–1000 µM isopropyl β‐D‐1‐thiogalactopyranoside (IPTG), is required to keep repressor LacI from its binding sites, which is a heavy cost burden in low‐value‐added products. So we propose a strategy to reduce the required dosage of...
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