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Vivek Maize Hybrid 9‐ a popular single‐cross hybrid developed by crossing CM 212 and CM 145 was released for commercial cultivation in India. The parental lines, being deficient in lysine and tryptophan, were selected for introgression of opaque‐2 allele using CML 180 and CML 170 as donor lines through marker‐assisted backcross breeding. The opaque‐2 homozygous recessive genotypes with >90% recovery...
With 3 figures and 5 tables
AbstractIdentification of candidate gene–based SSR markers is extremely useful in genetic enhancement of crop plants. A total of twenty‐four SSR loci were developed for the candidate genes of lysine and tryptophan metabolic pathways and for opaque2 (o2) modifiers. Of the 24 SSR loci, 23 yielded amplicons of expected size; however, fifteen were found polymorphic among the...
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