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The sodium hydrogen exchanger isoform one (NHE1) plays a critical role coordinating asymmetric events at the leading edge of migrating cells and is regulated by a number of phosphorylation events influencing both the ion transport and cytoskeletal anchoring required for directed migration. Lysophosphatidic acid (LPA) activation of RhoA kinase (Rock) and the Ras-ERK growth factor pathway induces cytoskeletal...
In this paper we demonstrate for the first time that human placenta contains a cytosolic phospholipase D (PLD) activity. This activity had a pH optimum of 7.0 and was stimulated by PIP 2 and inhibited by oleate. Furthermore, cytosolic PLD was stimulated by 30 μM GTPγS (6-14-fold) and by the small G proteins 1 μM mArf3 (2-fold) and 0.37 nM RhoA (2-fold). This is the first report to show RhoA...
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