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This study demonstrates that Aminoethylisothiourea (AET), a potent inhibitor of inducible nitric oxide synthase (NOS), is an irreversible competitive inhibitor of the diphenolase activity of mushroom tyrosinase when L-DOPA was assayed spectrophotometrically in vitro. The inhibition of diphenolase activity of tyrosinase by AET showed positive kinetic cooperativity which arose from the protection of...
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