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A method was developed to determine the Nɛ-carboxymethyl-lysine content in the muscle tissues of turbot using gas chromatography-mass spectrometric method. Turbot was selected as the research object, and homogenized muscle tissue samples of turbot defatted by chloroform/acetone were pretreated followed by reduction, acid hydrolysis, purifying and derivatization (esterification and acylation). The...
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