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Delayed pollination is widely used to overcome pre‐fertilization incompatibility, but its regulatory mechanisms are unclear. When Nicotiana tabacum was cross‐pollinated with pollen of N. alata, the incompatibility occurring in the basal 1/4 region of the style (pollinated at anthesis: 0‐day‐old pistil) was overcome by delayed pollination (of 6‐day‐old pistil), and the morphological changes and...
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