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Abstract Protoplasts were isolated from primary calli of barley (Hordeum vulgare L.), and an antibiotic (G418) resistance gene was introduced into these protoplasts using a polyethylene glycol (PEG) DNA uptake method. Sixty-four G418 resistant calli were obtained in nine experiments, and two plants were regenerated from these calli. NPTII ELISA and Southern analysis indicated that the G418 resistance...
Abstract: Nodule cultures were induced from shoot tips of aseptically grown Eucalyptus botryoides in a vertically rotated incubator. N-(2-chloro-4-pyridyl)-N-phenylurea (4PU) was a very effective cytokinin for induction of nodules in liqiud B5 basal medium supplemented with naphthalene acetic acid and 3% sucrose. The effective concentration of 4PU ranged from 0.2 to 0.5mg/l. The nodule had a very...
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