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Background: Since the first successful report of cloned mammals after nuclear transfer of somatic cells in sheep in 1997, cloned animals of various species have been produced. Generally, somatic cell nuclear transfer (SCNT) is performed with non-activated metaphase II (MII) oocytes as recipients, because activation of oocytes has adverse effects on the developmental potential of SCNT oocytes. Rat...
It is remarkable that mammalian somatic cell nuclei can form whole individuals if they are transferred to enucleated oocytes. Advancements in nuclear transfer technology can now be applied for genetic improvement and increase of farm animals, rescue of endangered species, and assisted reproduction and tissue engineering in humans. Since July 1998, more than 200 calves have been produced by nuclear...
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