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BEAMing (beads, emulsion, amplification, and magnetics) is a high sensitive emulsion digital PCR (polymerase chain reaction) technology. Target beads obtained via BEAMing can be used to determine the copy number of target DNA population. However, the existing beads counting process requires specialized instrument, which has increased the detection costs. Here we developed an easy and low cost beads...
An integrated microfluidic device with on-chip performance of cell lysis, DNA extraction and PCR amplification is achieved to realize the total analysis from cell lysis to DNA amplification on a single Polydimethylsiloxane (PDMS) chip. Using multilayered PDMS patterning and Solid Phase Extraction (SPE) techniques, DNA is extracted by magnetic beads (MBs) from the cell lysate prepared by a two-step...
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