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The effect of strengthening of interaction between single fluorescent probes and polymer matrix to the probes' dynamics is investigated using single molecule fluorescence defocus microscopy. By introducing multiple hydroxyl groups to the fluorescent probes (perylene diimide derivatives) which builds up hydrogen bonds between the probe and polymer matrix, the dynamics is discovered to be retarded....
Rotational motion of fluorophores chemically attached to polystyrene chain-ends in ultra-thin films on solid substrates was studied by single-molecule fluorescence de-focus microscopy. The collective feature of the rotational motion was found and evidenced by the sharp change of the population of fluorophores undergoing rotational motion within a very narrow temperature range (named as the changing...
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