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We developed and validated a total human DNA quantitation technique that simultaneously allows male DNA detection. This assay, called Amel‐Y, is a duplex Real Time PCR followed by HRM (high resolution melting) analysis using the intercalating dye SYTO9. Amel‐Y duplex produces two amplicons, one for the amelogenin gene (106/112 bp, female/male) and another (84 bp) corresponding to human Y chromosome‐specific...
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