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Post‐harvest storage is largely limited by fruit softening, a result of cell wall degradation. Pectin methylesterase (PE) (EC 3.1.1.11) is a major hydrolase responsible for pectin de‐esterification in the cell wall, a response to fruit ripening. Two major PE isoforms, PE1 and PE2, have been isolated from tomato (Solanum lycopersicon) pericarp tissue and both have previously been down‐regulated using...
Nectarine samples of the melting flesh ‘Snow Queen’ cultivar were harvested periodically around the commercial harvest date. A sharp decline in the ratio of insoluble to soluble cell wall materials preceded the melting-like decrease in fruit firmness, apparently arising from depolymerisation of polysaccharides bound tightly to the cell wall. Results suggest that part of the arabinose-rich side-chains...
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