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D-amino acid oxidase (DAO, EC 1.4.3.3) is one of the flavoenzymes in peroxisomes catalyzing the oxidation of D-amino acids to α-imino acids. By reverse transcription-polymerase chain reaction, DAO cDNA was cloned from the mRNA of zebrafish embryos. The deduced zebrafish DAO amino acid sequence revealed a 348-amino acid polypeptide containing two FAD-binding sites (amino acids positions 7G to 12G,...
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