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The suitability of using fluorescence spectroscopy to assess tryptophan (TRYP) depletion by quantifying the loss of natural TRYP fluorescence during metal-catalyzed oxidation (MCO) was assessed. To analyze the effect of the oxidation conditions, N-acetyl-l-tryptophanamide (NATA) solutions were oxidized in the presence of Cu 2+ and Fe 2+ (40 and 80μM), with and without 0.8mM H 2...
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