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l-threonine is an important amino acid that can be added in food, medicine, or feed. Here, the influence of glyoxylate shunt on an l-threonine producing strain Escherichia coli TWF001 has been studied. The gene iclR was deleted, and the native promoter of the aceBA operon was replaced by the trc promoter in the chromosome of TWF001, the resulting strainTWF004 could produce 0.39 g l-threonine from1 g...
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