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The design delivers the highlight of our review— using Pichia pastoris as whole‐cell catalyst for chemical production, exemplified by the oxidation of lactate. Lactate is diffused into the peroxisome and oxidized into pyruvate and H2O2 by the 2‐hydroxyacid oxidase. The co‐localized catalase removes accumulated H2O2 and regenerates O2 for further reaction. We adopted the pencil sketch effect for the...
With nearly three decades of development, Pichia pastoris (P. pastoris) has become a powerful eukaryotic protein expression system for the expression of thousands of proteins both on a laboratory and industrial scale. In addition, it has also been extensively used as a cell factory for the production of a variety of chemicals. This review summarizes the bottlenecks and solutions in achieving high‐level...
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