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Lymphocystis disease virus (LCDV) was detected by PCR-hybridisation in Artemia cysts and metanauplii, and the infectivity of these viral particles was confirmed by inoculation on SAF-1 cells. Viral genome and antigens have been detected by whole-mount in situ hybridisation (ISH) and immunofluorescence assay (IFA) in the digestive tract of nauplii hatched from LCDV-contaminated cysts, but not at the...
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