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With 1 figure and 7 tables
AbstractThe current study aims at producing doubled haploid Brassica rapa plants through isolated microspore culture. Genotypic differences in embryogenic response and regenerative ability of microspore‐derived embryos to plants were observed. Compared with the longer incubation period (72 h), heat shock treatment at 33°C for 24 or 48 h was more beneficial for microspore...
An efficient protocol to improve microspore embryogenesis and plant regeneration in Brassica rapa was established. The antiauxin p-chlorophenoxyisobutyric acid (PCIB) was used to enhance microspore embryogenesis and plant regeneration without an intervening callus phase. All the 4 tested genotypes responded positively to PCIB. The optimum concentration of PCIB application was found to be 40μM in NLN-13...
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