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All pyrroloquinoline quinone (PQQ)-containing dehydrogenases whose structures are known contain Ca 2+ bonded to the PQQ at the active site. However, membrane glucose dehydrogenase (GDH) requires reconstitution with PQQ and Mg 2+ ions (but not Ca 2+ ) for activity. To address the question of whether the Mg 2+ replaces the usual active site Ca ...
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