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Osteoclasts are multinucleated giant cells that differentiate from precursors of the monocyte-macrophage lineage. We used receptor activator of NF-kappa B ligand (RANKL) and macrophage colony stimulating factor (M-CSF) to differentiate authentic human osteoclasts from peripheral blood mononuclear cells (PBMCs). We previously described a series of genes that are strongly regulated by RANKL. Here, we...
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