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A simple microfluidic device which can perform antimicrobial susceptibility testing (AST) of combined antibiotics against antibiotics-resistant bacteria has been presented. A new approach to determine the fractional inhibitory concentration (FIC) index of two antibiotics by performing broth dilutions automatically was proposed and its performance was highly consistent with manual operation. The experimental...
This study presents an integrated microfluidic system which can identify the vancomycin resistant gene (vanA) from live hetero-bacteria automatically. In this study, a new approach to diagnose the vanA gene from live hetero-bacteria by using ethidium monoazide (EMA) and loop-mediated isothermal amplification (LAMP) was proposed and its feasibility was tested and verified. In addition, an integrated...
Periprosthetic joint infection (PJI) is a devastating complication after arthroplasty. Two-stage reimplantation procedure is the most common practice to prevent the reoccurrence of PJI. However, it should be assisted by a rapid, accurate and comprehensive bacteria detection tool. In this study, a new integrated microfluidic system capable of performing sample pre-treatment, a nanogold detection to...
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