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Intracellular Mg 2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg 2+ regulation, we visualized intracellular Mg 2+ using the fluorescent Mg 2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein...
Excess administration of glutamate is known to induce Ca2+ overload in neurons, which is the first step in excitotoxicity. Although some reports have suggested a role for Mg2+ in the excitotoxicity, little is known about its actual contribution. To investigate the role of Mg2+ in the excitotoxicity, we simultaneously measured intracellular Ca2+ and Mg2+, using fluorescent dyes, Fura red, a fluorescent...
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