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Hemocytes from the red swap crayfish Procambarus clarkii were classified into three subpopulations: hyalinocytes (H), semigranulocytes (SG) and granulocytes (G) by morphologic observation and flow cytometry (FCM). The primary hemocyte culture was then established for studies on the in vitro propagation of Spiroplasma eriocheiris isolated from naturally infected P. clarkii. Grace's insect medium supplemented...
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