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Gespleißt: Eine N‐Methylgruppe wurde an der zu spaltenden Peptidbindung in einem semisynthetischen Intein‐Vorläuferprotein eingeführt. Diese chemische Modifikation bewirkte Thioesterbildung und Proteinspleißen in Abwesenheit des sonst essentiellen Block‐B‐Histidins. Das Ergebnis demonstriert die Rolle des Histidins in der Grundzustandsdestabilisierung und schließt andere diskutierte Rollen dieser...
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