The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
A newly fluorescence probe for detection of Serotonin based on a Mn-doped ZnS quantum dots@silica nanoparticle@molecularly imprinted polymers (QDs@SiO2@MIPs) was successfully created. QDs@SiO2@MIPs were characterized by scanning electron microscope, transmission electron microscope, Fourier transform infrared spectroscopy, UV spectroscopy, X-ray powder diffraction, and fluorescence spectroscopy. A...
Fe3O4@MnO2 magnetic composite microsphere with hierarchical shells structure has been synthesized through a facile two-step hydrothermal reaction for ultra-trace arsenic enrichment. Scanning electron microscopy and transmission electron microscopy images clearly indicated that the as-synthesized material is a porous hydrangea-like morphology, as well as the size of the composite microspheres and the...
A sample treatment technique based on a duel frequency ultrasonic device for enzymatic digestion of rice is reported. The ultrasonic device combines a high intensity ultrasonic probe and the temperature control function of ultrasonic water bath, which can effectively extract cadmium from rice within only 160 s under the optimized conditions. Compared with the traditional ultrasonic assisted enzymatic...
Colorimetric assay is a powerful tool for detection of tumor markers with the outstanding advantages of visualization and simple analytical instruments. Herein, a simple and fast chromogenic reaction was developed based on Ag3PO4/Ag nanocomposite synthesized by one-step chemical bath method. And the Ag3PO4/Ag nanocomposite owned the ability of oxidizing of TMB under the acidic condition due to the...
Alkaline phosphatase (ALP), one of the important hydrolases, is associated with the progress of many diseases as a well-defined biomarker. Fluorescence imaging of ALP in living organisms is of great importance for biological studies. However, in vivo detection of ALP remains a great challenge because current fluorescent probes show short excitation and emission wavelength, which are not desired for...
This work illustrates an accurate method for determination of Se by electrolytic hydride generation technique based on a novel carbon paste electrode for sample introduction combined with atomic fluorescence spectral analysis. The studies show that Se(IV) can be converted efficiently to SeH2 on an L-cysteine modified carbon paste electrode (CMCPE), which has never been reported before. Significantly,...
Visual detection of tumor markers with accuracy and selectivity exhibits significant advantages for cancer early diagnosis due to its low-cost and simple analytical instruments. Herein, a highly photosensitive colorimetric immunoassay for carcino embryonie antigen (CEA, as a model) detection based on Cu2+ doped Ag-AgI nanocomposite was constructed. First, Cu2+ doped Ag-AgI nanocomposite (Cu2+/Ag-AgI)...
The monitoring of K+ is very important and emergency because of their unique relationship in various disease diagnosis and treatment. G-quadruplex analogue is a classical recognition unit for K+ detection and has been widely applied in K+ relevant research. Common fluorescent dyes were employed for design of G-quadruplex structure-based K+ probes which suffered from the aggregation-caused quenching...
A novel non-chromatographic speciation technique for ultra-trace arsenite [As(III)] and total arsenic (tAs) in Chinese herbal medicine (CHM) is developed and validated by electrolytic hydride generation (EHG) coupled with atomic fluorescence spectrometry (AFS). The studies show that As(III) can be converted efficiently to AsH3 on an L-cysteine modified carbon paste electrode (CMCPE), which has never...
This work describes a novel non-chromatographic approach for the accurate and selective determining As species by modified graphite electrode-based electrolytic hydride generation (EHG) for sample introduction coupled with atomic fluorescence spectrometry (AFS) detection. Two kinds of sulfydryl-containing modifiers, l-cysteine (Cys) and glutathione (GSH), are used to modify cathode. The EHG performance...
In this work we use a water-soluble cationic perylene derivative (compound 1) as the G-quadruplex (G4) structure fluorescence indicator to construct a fluorescent biosensor for simple, rapid and label-free detection of Pb2+. In the absence of Pb2+, strong electrostatic interactions between compound 1 and the G-rich DNA probe (PW17) induced the aggregation of compound 1 and resulted in the fluorescence...
DNAzyme-based catalytic beacons have been widely studied for both in vivo and in vitro molecular detection. However, only a few label-free catalytic beacons with excellent analytical performance have been reported so far. In this work, by combining a catalytic DNAzyme for amplified sensing through enzymatic turnover with cleavage-induced G-quadruplex formation, a label-free DNAzyme biosensor was developed...
Intracellular pH plays a pivotal role in various biological processes. In eukaryotic cells, lysosomes contain numerous enzymes and proteins exhibiting a variety of activities and functions at acidic pH (4.5–5.5), and abnormal variation in the lysosomal pH causes defects in lysosomal function. Thus, it is important to investigate lysosomal pH in living cells to understand its physiological and pathological...
Toxaphene, which is a broad spectrum chlorinated pesticide, is a complex mixture of several hundred congeners, mainly polychlorinated bornanes. Quantifying toxaphene in environmental samples is difficult because of its complexity, and because each congener has a different response factor. Toxaphene chromatograms acquired using one-dimensional gas chromatography (1DGC) show that this technique cannot...
Development of fluorescent probes for Hg 2+ has become a hot topic in modern chemical research due to its high toxicity. In this paper, we for the first time report the synthesis and application of a thioether spirocyclic rhodamine B derivative (TR) as an efficient fluorescent probe for Hg 2+ . TR was synthesized using a simple procedure under mild condition. By employing a thioether...
A simple capillary array IEF device was developed for high resolution and micropreparative separation of trace amounts of proteins. Based on quasi-chip-scale manufacturing, the specific capillaries (600μm i.d., 1200μm o.d. and 20mm length) were integrated with the miniaturized polymethyl-methacrylate electrode trays. Electroosmotic flow was suppressed effectively by modified cross-linked polyacrylamide...
A new and facile strategy using double-stranded DNA-copper nanoparticles (dsDNA-Cu NPs) as fluorescence reporters for the highly sensitive and selective detection of l-histidine was demonstrated. In the dsDNA-Cu NPs-based sensing system, the fluorescence was quenched considerably upon the addition of l-histidine. Under the optimized experimental conditions, the probe exhibits excellent performance...
A novel redox-active magnetic nanostructure was synthesized by using a wet chemical method for high-efficiency electrochemical immunoassay of carcinoembryonic antigen (CEA, as a model analyte). The nanostructures based on the combination of a magnetic nanocore, a layer of electroactive poly(o-phenylenediamine) (PPD), and a silver metallic shell displayed good adsorption properties for the attachment...
The fluorescence spectra of berberine, palmatine, jatrorrhizine, and coptisine in ionic liquids were studied and found to increase significantly in ionic liquids, with [C 8 MIM][PF 6 ] having the greatest increase. Further studies showed that these drugs could be extracted from an aqueous solution by [C 8 MIM][PF 6 ] using the temperature-assisted ionic liquid dispersive...
In this study we firstly report a new electrolytic cold vapor generation system for mercury determination on Pt/Ti cathode in the presence of organic acid catholyte. Comparing with the traditional inorganic acid, formic acid increased the signal intensity of Hg vapor from electrolytic generation on Pt cathode and reduced the impact of cathode erosion on the stability of signal intensity. Moreover,...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.