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We introduce concentration‐dependent number and brightness (cdN&B), a fluorescence fluctuation technique that can be implemented on a standard confocal microscope and can report on the thermodynamics of membrane protein association in the native plasma membrane. It uses transient transfection to enable measurements of oligomer size as a function of receptor concentration over a broad range, yielding...
Frei beweglich: Nach einem kürzlich vorgestellten Modell für die Spannungskontrolle in Kaliumkanälen stehen die vier Argininreste der S4‐Helix (links), eines Spannungssensors, in direktem Kontakt mit den Membranlipiden und bewegen sich während des Gating‐Prozesses in den Kohlenwasserstoffkern der Membran. Hier wird gezeigt, dass die physikalischen Eigenschaften der isolierten S4‐Sequenz (rechts) zur...
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