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Helix 7 in the Cry4Ba-pore-forming domain contains conserved Tyr 249 and Phe 264 that are crucially involved in mosquito-larvicidal activity. We have now characterized lipid-induced conformation of a 27-residue Cry4Ba-α7 peptide in phospholipid membranes using ATR-FTIR and hydrogen/deuterium (H + /D + ) exchange experiments. ATR-FTIR results showed that conformation...
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