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A chemo-biotechnological approach is reported for the synthesis of TMG-chitooligomycins, CO-n (NMe 3 ). Their abilities to inhibit β-N-acetylhexosaminidases (HexNAcases), from Aspergillus oryzae (AoHex, fungi), Canavalia ensiformis (CeHex, plant) HexNAcases and a chitobiase from Serratia marcescens (SmCHB, bacteria) were studied and compared with their precursors CO-n (N). CO-n (NMe 3...
Large amounts of globotriose (Galα-4Galβ-4Glc) are shown to be produced by the high cell density culture of an Escherichia coli strain over-expressing the Neisseria meningitidis lgtC gene for α-1,4-Gal transferase. The strain which was devoid of both α and β galactosidase activity was fed with glycerol as the energy and carbon source and with lactose as precursor for globotriose synthesis. After complete...
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