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A simple assay for monoacylglycerol hydrolysis suitable for high-throughput screening is described. The assay uses [ 3 H]2-oleoylglycerol as substrate, with the tritium label in the glycerol part of the molecule and the use of phenyl sepharose gel to separate the hydrolyzed product ([ 3 H]glycerol) from substrate. Using cytosolic fractions derived from rat cerebella as a source of...
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