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HSK7653 is a novel super long‐acting dipeptidyl peptidase‐4 inhibitor, which is promising for the treatment of type 2 diabetes mellitus with the twice‐monthly dosing regimen. In this article, a robust and sensitive HPLC coupled with tandem mass spectrometry method for determining the concentration of HSK7653 in human plasma and urine was developed and validated for the first time. Plasma and urine...