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Immune responses induced by a nasal influenza vaccine with a mutant cholera toxin (CT112K), known to be a safe adjuvant, were characterized in BALB/c mice to confirm the most suitable regimen of this vaccine for humans. Mice received a primary intranasal administration of the adjuvant (0.1μg)-combined PR8 vaccine (0.1μg) and a secondary administration of the PR8 vaccine alone (0.1μg) 4 weeks later...
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