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Immobilisation of β-glucosidase, isolated from Aspergillus niger, by entrapment in both calcium alginate and polyacrylamide gels was studied. A retention of 66% of initial activity was observed in the alginate beads prepared with 3% (w/v) alginate, 0.2 m CaCl 2 and 1 h of treatment. The maximum β-glucosidase activity in polyacrylamide gels ( 55%) was achieved in gels prepared with 20%...
Amorphous cellulose induced the synthesis of endoglucanase in Trichoderma reesei to a greater extent than did cellobiose, lactose, sucrose or other commercial celluloses. In contrast, none of these carbohydrates was able to induce significant levels of endoglucanase activity in Aspergillus niger or Pseudomonas pickettii. Both endoglucanase and β-glucosidase activities were found in cell wall, cell-free...
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