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A novel self-assembled glucose biosensor based on graphene oxide (GO) was constructed by using 1-pyrenebutyric acid–N-hydroxysuccinimide ester (PANHS) as linking molecular. The stepwise self-assembly process was performed for PANHS anchoring in N,N-dimethylformamide (DMF) solvent and the further glucose oxidase (GOD) binding in aqueous solution, respectively. The molecular interactions and the morphologic...
Protein kinase Cζ (PKCζ) plays a critical role in cancer cell chemotaxis. Upon activation induced by epidermal growth factor (EGF) or chemoattractant SDF-1α, PKCζ redistributes from cytosol to plasma membrane. Based on this property, we developed a rapid cell-based assay for inhibitors of ligand-induced PKCζ activation. PKCζ green fluorescent protein (GFP) was transfected into human breast cancer...
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