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A quantitative rapid detection method based on loop‐mediated isothermal amplification has been developed for red‐spotted grouper nervous necrosis virus (RGNNV). The nested polymerase chain reaction (PCR) assay is the mainstream inspection of the brooder in the hatchery. In this study, a real‐time loop‐mediated isothermal amplification (LAMP) method has been applied for RGNNV detection, known as a...
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