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A Thiol-functionalized silica hollow nanospheres(denoted as SiO2-SH NSs) were prepared through a hydrothermal route. The SiO2-SH NSs were conjugated with glutathione group (denoted asGSH) to afford SiO2-GSH NSs. The as-prepared SiO2-GSH sample has hollow structure and exhibits an average diameter of about 45nm and a wall thickness of 10nm. The SiO2-GSH NSs were used to separate three kinds of GST-tagged...
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