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An amperometric urea-sensing electrode was prepared by immobilizing a tri-enzyme system of pyruvate oxidase (PyOx), pyruvate kinase (PK) and urea amidolyase (UA) on a polydimethylsiloxane (PDMS)-coated electrode. UA catalyzes the adenosine-5'-triphosphate (ATP)-dependent hydrolysis of urea to generate adenosine-5'-diphosphate (ADP); PK, the phospho-transferring reaction between ADP and phosphoenolpyruvate...
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