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We have analyzed a possible role of mitogen-activated protein kinase (MAPK) and activator protein-1 (AP-1) in the regulation of FSH-induced tissue type plasminogen activator (tPA) production in granulosa cells (GCs) prepared from DES-treated immature rats; Treatment of the cells in the presence of FSH with MAPK inhibitors, such as UO126 or SB203580, significantly decreased the FSH-induced tPA production,...
Our previous studies have demonstrated that tissue type plasminogen activator (tPA) might be involved in matrix degradation of blood-testis barrier in rat. In this study, we have further investigated the effect of testosterone (T) on tPA production in rat Sertoli cells. Our results showed that Sertoli cells isolated from rat testes at various ages in vitro secreted tPA in an age-dependent manner....
We have examined the expression of MMP-2, TIMP-1, and TIMP-3 mRNA at the implantation site of rhesus monkeys during early stage of pregnancy using in situ hybridization and Northern blot analysis. The results indicate that MMP-2 mRNA was mainly localized in the chorinoic villi and epithelial plaque, suggesting that MMP-2 may be involved in the process of epithelial plaque and trophoblast invasion...
We investigated the possible role of Hsp70-2 in germ cell apoptosis induced by heat stress in monkey unilateral cryptorchid testis. The study focused on in situ analysis of the testicular cell DNA fragmentation and on the possible relationship between Hsp70-2 expression and germ cell apoptosis. The TUNEL result showed that most of the germ cells were labeled in the cryptorchid testis on d 5 after...
A hormone response element, CNTFRα-NBRE (5′-AAAGGTCA-3′) has been identified in the fifth intron of the α component of ciliary neurotrophic factor receptor gene (CNTFR-I5) for the human TR3 orphan receptor (TR3). A specific binding between in vitro expressed TR3 and CNTFRα-NBRE was demonstrated by using electrophoretic mobility shift assay. A reporter gene assay using chloramphenicol acetyltransferase...
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