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A new water-soluble cationic porphyrin derivative m-TMPyEOPP (5,10,15,20-tetrakis{4-[2-(O-(N-methylpyridyl)ethoxyl]oxyphenly}porphyrin) has been designed, synthesized, and fully characterized. This porphyrin derivative combines the advantages of two cationic porphyrins used in G-quadruplex recognition and stabilization studies, i.e., the widely investigated G-quadruplex ligand TMPyP4 and TMPipEOPP...
To promote application of strand-displacement amplification (SDA) techniques in biosensing, a label-free, real-time monitoring strategy for isothermal nucleic acid amplification reactions was designed. G-quadruplex structures were introduced into SDA products using specific recognition of G-quadruplexes by the fluorogenic dye thioflavin T. Performance was good for real-time monitoring of traditional...
Triphenylmethane (TPM) dyes normally render rather weak fluorescence due to easy vibrational deexcitation. However, when they stack onto the two external G-quartets of a G-quadruplex (especially intramolecular G-quadruplex), such vibrations will be restricted, resulting in greatly enhanced fluorescence intensities. Thus, TPM dyes may be developed as sensitive G-quadruplex fluorescent probes. Here,...
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