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Isogenic Y. pestis strains with a single mutation in 14 genes of lipopolysaccharide (LPS) biosynthetic pathways were constructed. Using high-resolution electrospray ionization mass spectrometry, the full LPS structures were elucidated in each mutant, and the sequence of monosaccharide transfers in the assembly of the LPS core was inferred. Truncation of the core decreased significantly the resistance...
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