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Surface-enhanced Raman scattering (SERS) spectra, which can provide large information about trace amount of chemical and biological species have been widely performed as a well-established tool in complex biological system. In this work, coomassie brilliant blue (R-250) with high affinity to proteins and high Raman activity was employed as a Raman reporter to probe prion protein (PrP) through a dual-aptamer...
In this contribution, an organic small molecule (OSM)-participating interaction between its aptamer and graphene oxide (GO) is investigated by taking coralyne as an example. Based on their interactions, a simple, rapid, highly sensitive and selective fluorometric method for the detection of coralyne is developed. GO can effectively quench the fluorescence of dye-labeled aptamer, while stronger binding...
An aptamer-participated haprin structure was designed by employing cellular prion protein (PrP C ) as a model protein, and thus an aptamer-mediated turn-on fluorescence assay for proteins was developed in this contribution. The designed aptamer-participated haprin structure consists of three segments. Namely, an aptamer sequence located in the loop, three guanine bases at 3′-terminal, and...
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