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The TEF4 gene of the non-saccharomyces yeast Yarrowia lipolytica encodes an EF1Bγ protein with structural similarity to the glutathione transferases (GSTs). This 1203bp gene was cloned, over-expressed in Escherichia coli, and the recombinant protein characterized. DNA sequencing of the cloned gene agreed with the recently completed Y. lipolytica genome and showed 100% identity to a previously reported...
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