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Tissue engineered skin constructed by cultured seed cells provides a new way to accelerate skin wound healing and improve the quality of tissue repair. Conventional models with dermal scaffold material based on collagen hydrogels, however, have poor stability and unsatisfactory longevity. Here, we describe an improved acellular amniotic membrane as a scafford with epidermal stem cells for tissue engineering...
The amniotic membranes have unique properties including transparency, stimulatory effect on cell proliferation and lack of immunogenicity, which may have clinical utility for cell delivery. In this chapter, we investigate the possibility of amniotic membranes loaded with bone marrow mesenchymal stem cells for healing of deep burn wound. The mesenchymal stem cells derived from human bone marrow were...
Tissue engineered skins have the potential to overcome the limitations of present large area of skin replacements. To achieve this, we investigated the effect and fate of tissue engineered skins comprising human epidermal stem cells and acellular amniotic membrane after their transplantation to full-thickness skin defect wound, so as to lay a foundation for clinic application. The human epidermal...
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