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Meranzin hydrate (MH), a compound derived from Chaihu–Shugan–San (CSS), displayed potent preclinical antidepressant functions. In this study, an LC–MS/MS method was developed and validated to quantify the MH in rat hippocampus and plasma. Tert-butyl methyl ether and irbesartan were used as the extraction reagent and the internal standard (IS), respectively. Chromatographic separation was achieved...
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